| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000433 |
| Chromosome: | chromosome 3 |
| Location: | 7350373 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206600 | AAD1 | Acetohydroxyacid dehydratase; (1 of 1) 4.2.1.9 - Dihydroxy-acid dehydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGCCAGCCAGCAACTTGTCGCGCACTCCTGCACTGCCGTTGCAAAT |
| Internal bar code: | CTAAGGGCGTTGTGTGGAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2674 |
| LEAP-Seq percent confirming: | 98.9011 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGGTGACTTGACGGTTA |
| Suggested primer 2: | CATTTGGCTTGACCCAGCAC |