Insertion junction: CLIP2.000451_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000451
Chromosome:chromosome 12
Location:2230523
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g508750 LHCA2 Light-harvesting protein of photosystem I; (1 of 24) IPR001344//IPR022796//IPR023329 - Chlorophyll A-B binding protein, plant // Chlorophyll A-B binding protein // Chlorophyll a/b binding protein domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCAATGATCATGGGCAGCACGCACAGCACGGCCACGGTCACGGCGGTC
Internal bar code:TATGGCGTCCTGTCACACACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3390
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTCCCCTTGTGTTTGTG
Suggested primer 2:CGCATGCTCTCCATAGACGT