| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000474 |
| Chromosome: | chromosome 16 |
| Location: | 7762861 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687406 | POB20 | Proteome of basal body 20 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCGCACCCGCGCCTCCAGCCCCGCCCGGTCCGCCCGCTGCGACATGG |
| Internal bar code: | ATTCCGGTCGTCCGCGCCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6743 |
| LEAP-Seq percent confirming: | 98.9899 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAGGCGCTGTAGATGTT |
| Suggested primer 2: | GGCCTTCGTGAAAAACACCC |