Insertion junction: CLIP2.000475_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.000475
Chromosome:chromosome 6
Location:1334465
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g259100 POB30 Proteome of basal body 30 CDS
Cre06.g259150 EFG8 (1 of 1) PTHR23115//PTHR23115:SF155 - TRANSLATION FACTOR // SUBFAMILY NOT NAMED; Translation elongation factor Tu family protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGGGGAAATGTACTACAGGGCAATAGAGAATGCTGATTCTAAAGCCAG
Internal bar code:AGTTCGACGGTTCGAACGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1560
LEAP-Seq percent confirming:93.9394
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGCAGTCCCCACATGAAC
Suggested primer 2:TTCCCGTAACCTCGAAGCAC