Insertion junction: CLIP2.000498_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.000498
Chromosome:chromosome 3
Location:5381645
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g183550 (1 of 3) PTHR15160//PTHR15160:SF4 - VON HIPPEL-LINDAU PROTEIN // PROTEIN F23H11.5 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCGGGCGATACTGCAACCAACATCTACACGCAGGTGGGCAAGGGGAAG
Internal bar code:TTTGCAGAAGATCGAGGGGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1764
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGTTGTGGAGCTTGTGC
Suggested primer 2:CGTTTGCACTTGGACTTGGG