Insertion junction: CLIP2.000499_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.000499
Chromosome:chromosome 16
Location:2884090
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g663100 ALG7 (1 of 1) 2.7.8.15 - UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase / N-acetylglucosamine-1-phosphate transferase; N-acetylglucosamine phosphostransferase 3'UTR
Cre16.g663150 (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGCTCCCACACTCTCTCTTCCGCTGGTCCTCCTACTCCTAAAGCGCC
Internal bar code:CAGCGTTATCATGTGAGTCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:798
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTACGCATAGCAGGATCG
Suggested primer 2:TGCAGGAGGAGCTGGAAAAG