| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000514 |
| Chromosome: | chromosome 1 |
| Location: | 3083249 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019550 | ankryin repeat containing protein; (1 of 2) PTHR24128:SF17 - E3 UBIQUITIN-PROTEIN LIGASE XBAT32 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCTGTTCTCGCCCGTTCACTACGTTTGTTGGGAAGTCTGGTCACCTT |
| Internal bar code: | ACTAATCCAAAACAAGCGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3024 |
| LEAP-Seq percent confirming: | 82.6087 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACCTAGACTTTTGGCT |
| Suggested primer 2: | CCCAAAGCACTCACTTGCAC |