| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000522 |
| Chromosome: | chromosome 16 |
| Location: | 3257655 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666050 | CPLD49,SCD1 | (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP); Cytochrome b6f biogenesis protein | 5'UTR |
| Cre16.g666100 | (1 of 1) K14168 - cytoplasmic tRNA 2-thiolation protein 1 (CTU1, NCS6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGCCCTGACCGGCGTCGGCCTTGCTGTTTACATTTCGCCCAGGCGC |
| Internal bar code: | TCTAAGATACCTATGGGACCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5177 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGTATCGCGCGTTGAG |
| Suggested primer 2: | AAAGCCCGCTTTGGATAGCT |