Insertion junction: CLIP2.000532_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000532
Chromosome:chromosome 12
Location:2231235
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g508750 LHCA2 Light-harvesting protein of photosystem I; (1 of 24) IPR001344//IPR022796//IPR023329 - Chlorophyll A-B binding protein, plant // Chlorophyll A-B binding protein // Chlorophyll a/b binding protein domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTCCGGTCCGAGCCGCACGCTGCGCTCAAACCTGACACGGAGCAACA
Internal bar code:GACGCGGTATAACTAGTACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3810
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCTGCCCATGATCATTG
Suggested primer 2:GCCATCGATTAGAGTGGCGA