| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000555 |
| Chromosome: | chromosome 12 |
| Location: | 1695042 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486250 | ARL6,ARFA1A,FAP333 | (1 of 2) PTHR11711:SF181 - ADP-RIBOSYLATION FACTOR A1F; Flagellar Associated Protein 333 | 3'UTR |
| Cre12.g486300 | PSAL,PSAL1 | (1 of 1) K02699 - photosystem I subunit XI (psaL); Photosystem I reaction center subunit L | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTAGAAACCACGTGCCCGGCCGCTCTGACACGCACAGCACCTGCCTTG |
| Internal bar code: | AGATATCCATTAGAATCTAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3340 |
| LEAP-Seq percent confirming: | 65.6 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 125 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGATGGGGAAAGAGCTT |
| Suggested primer 2: | CCCCCTCCTACAACTCGAGA |