| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000559 |
| Chromosome: | chromosome 14 |
| Location: | 1479013 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617450 | HSP22E | Heat shock protein 22E; (1 of 4) PTHR11527//PTHR11527:SF128 - SMALL HEAT-SHOCK PROTEIN HSP20 FAMILY // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCGGTCGATGCTGCCGGTCCGACCTTCGCGGCAGGGCGCCGTGACGC |
| Internal bar code: | GCTCTAGATGAGTACCGGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 7.40741 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGGGAAAGAAGGGAAGG |
| Suggested primer 2: | GGTGGAAGCGACTGACTTCA |