| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.000573 |
| Chromosome: | chromosome 2 |
| Location: | 8229149 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143635 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATTTAAGACGAAAAGAGGTTCGGTGGGTTCGCCATGTCAGGCCGTGTC |
| Internal bar code: | CGTTCCACAACAACTTTGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2333 |
| LEAP-Seq percent confirming: | 80.5556 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTCACCCATGCATCAGT |
| Suggested primer 2: | CCCGTTTCCAAGCACTGTTG |