| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.000581 |
| Chromosome: | chromosome 3 |
| Location: | 4129778 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACGTCTCTCTTCGTGCCTGCCTCATTGCACCCCACGAGTTCACCCCG |
| Internal bar code: | GGATCCGGCCTCATGTGCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4123 |
| LEAP-Seq percent confirming: | 12.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGAGATGCATCGTCTGTA |
| Suggested primer 2: | GCAGCTGATGGTTTATGGCG |