Insertion junction: CLIP2.000589_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000589
Chromosome:chromosome 3
Location:8358470
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g199000 PHOT,PHT1,PHOT1 (1 of 1) PTHR24351//PTHR24351:SF76 - RIBOSOMAL PROTEIN S6 KINASE // PROTEIN TWIN LOV 1; Phototropin 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGTCACGGACCGCTACGCGCCCGCAGCCGCGCTCGCGGCGGACCCAC
Internal bar code:TGTTCCTCGGAATTCTGACGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:421
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGCGGCATACTTTCGTCG
Suggested primer 2:TTCGGGAGGATAAGGAGCCA