| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000592 |
| Chromosome: | chromosome 1 |
| Location: | 2719485 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016514 | DLD2 | (1 of 1) PTHR22912//PTHR22912:SF142 - DISULFIDE OXIDOREDUCTASE // SUBFAMILY NOT NAMED; Dihydrolipoamide dehydrogenase 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGATGTCCTGGGGGGCCGGGCGGGGGGCGAAGAGGTAGCGAGGTGGG |
| Internal bar code: | TATCACCGGTCACCATGAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCCGAAATGCACCGAGT |
| Suggested primer 2: | CCCCCACACAATCTGCATCT |