| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000596 |
| Chromosome: | chromosome 2 |
| Location: | 4866162 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109550 | HSD1,AHD3 | Homoserine dehydrogenase; (1 of 2) K12524 - bifunctional aspartokinase / homoserine dehydrogenase 1 (thrA) | 5'UTR |
| Cre02.g109600 | INM1,IPP3 | Inositol monophosphatase; (1 of 2) K01092 - myo-inositol-1(or 4)-monophosphatase (E3.1.3.25, IMPA, suhB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACTCCGTCACCCTGTCAAGGGCTGTGTCCTCTGCCTCACGCACTGCC |
| Internal bar code: | ATAAGTAATTCTAGTGCCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3570 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGCTAGATGCACAACATG |
| Suggested primer 2: | GCCACCACGTTGAACTTGTC |