| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000615 |
| Chromosome: | chromosome 17 |
| Location: | 6733533 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746247 | (1 of 1) K11430 - histone-lysine N-methyltransferase EZH2 (EZH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTCACGGAATTCACAGTAGATGTATCATGATTCTAATTCTCCATTCC |
| Internal bar code: | GCGAAATCACAGAGGGTGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 96 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTTCCTACTGGACGTCG |
| Suggested primer 2: | CCAGGAAGTAAGCCACTGGG |