Insertion junction: CLIP2.000624_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.000624
Chromosome:chromosome 6
Location:1598486
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g261400 DTH1 Delayed in Triacylglycerol Hydrolysis 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTGGGCGGATGGTGAAGTGTCTGCGTGCCGCCTGTGCTCCCTGCAGAT
Internal bar code:GTGTCTAGAACGGCCCTACATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2119
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTTCCAAATATGCCGG
Suggested primer 2:TCGCAAGGTGGTTCTCTGAC