Insertion junction: CLIP2.000627_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000627
Chromosome:chromosome 5
Location:1827285
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g235500 KIN7A,KIN7-1 Kinesin motor protein; (1 of 1) PTHR24115//PTHR24115:SF70 - FAMILY NOT NAMED // KINESIN-RELATED PROTEIN 11 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCGCGCGGCGACCACAACCGCAAGGTGTCCGCCACCGCCTTCAATGAG
Internal bar code:GCACGGTGGGCATGCTGGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2596
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCGTGCAAATCCCTTGTG
Suggested primer 2:TACTGCGCTGCTGTGATTGA