| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000632 |
| Chromosome: | chromosome 6 |
| Location: | 4271930 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278255 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAAGATGCAGTCAAGTAGGGGGTTGGGGTGAAGCGGTGAAGGATGTT |
| Internal bar code: | ATTTCTTGGCGCGCGGATCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1184 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGGGAGCAGGCATAAGA |
| Suggested primer 2: | TATGGCCCAAGAGTCGAGGA |