Insertion junction: CLIP2.000653_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000653
Chromosome:chromosome 1
Location:2722039
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g016514 DLD2 (1 of 1) PTHR22912//PTHR22912:SF142 - DISULFIDE OXIDOREDUCTASE // SUBFAMILY NOT NAMED; Dihydrolipoamide dehydrogenase 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTGTACCAACGCCGCCGTCTGCATGTAACGCATTGCCATGCCGCTAC
Internal bar code:CCAAGGATTGACCGCGGTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:148
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAACGACTCGCTTATGCAGC
Suggested primer 2:ACCGGCTGTACCTTCTGTTG