| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000653 |
| Chromosome: | chromosome 10 |
| Location: | 1773871 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430600 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGACGATCCTGGAGCGAGTTGAGGTTGAGGCCAACGGCCAGATCATT |
| Internal bar code: | GTGTATTGAGCAAATGCTGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2583 |
| LEAP-Seq percent confirming: | 52.6316 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAAGGGCAGTTCGTACA |
| Suggested primer 2: | GTACCGACGGATTGCTAGGG |