| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000667 |
| Chromosome: | chromosome 10 |
| Location: | 2231858 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433750 | PAP1 | Class-I Nucleotidyl-transferase 1, nuclear; (1 of 1) K14376 - poly(A) polymerase (PAP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGACGCGAGCGCTGCGCCCGCCGCAGCGCTGCCGGCGCAGCAGCCAGC |
| Internal bar code: | CTTGAGTGGTAATACACGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4406 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAAGAGCAATCCAGCGC |
| Suggested primer 2: | TCCAAGGACGTCGTTAAGGC |