| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000677 |
| Chromosome: | chromosome 17 |
| Location: | 6727645 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746247 | (1 of 1) K11430 - histone-lysine N-methyltransferase EZH2 (EZH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGTGGCTGGGCGGGGGGTGTTGCAGCTGGCGTCTCCCTCTTCACCA |
| Internal bar code: | GATTGGCCGGAATTTGTAGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 90 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTGTGCTCAAAGAAACA |
| Suggested primer 2: | AAGGGCGGATGATGTGAGTG |