| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.000683 |
| Chromosome: | chromosome 1 |
| Location: | 3479214 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022681 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGTACACAACGCCCGTAAGCCCCCACCCCACACACGCACACAGGCAT |
| Internal bar code: | TACCTTATTAGTTAGCAACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5396 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCAGTGGGCCATAGAAG |
| Suggested primer 2: | CCGACCATTACAACCAGGCT |