| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000684 |
| Chromosome: | chromosome 6 |
| Location: | 5019813 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281800 | (1 of 6) PF09353 - Domain of unknown function (DUF1995) (DUF1995) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAAGCGGAACTCCGTCTGGATGCGGTCAAAGATGGACTGCACACGGG |
| Internal bar code: | GTAATACGATGGGGATGGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5232 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 112 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTCCCTGCTGTGCCTAC |
| Suggested primer 2: | GCAAAATGCATAGGGCCGAG |