Insertion junction: CLIP2.000687_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.000687
Chromosome:chromosome 5
Location:1471241
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234651 (1 of 2) PTHR11122:SF12 - ALDOSE 1-EPIMERASE FAMILY PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATGCAGCTGGCGACGCTGCGCGAACGCGGAGGAGGAGGGCACATGTAC
Internal bar code:TATCGAATTTATTTTTTGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4288
LEAP-Seq percent confirming:96.3855
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCAAGTTTGTGTCAGGG
Suggested primer 2:CGCTAGCTTCGCACACAATC