| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000696 |
| Chromosome: | chromosome 3 |
| Location: | 2806761 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161800 | MDN1 | (1 of 1) K14572 - midasin (MDN1, REA1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACTCATCCCTGCATTAGGGTTGTGCCAGGTGCTAATGAATGCCTGGA |
| Internal bar code: | ACGCATGGGGTATAGGAAGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1456 |
| LEAP-Seq percent confirming: | 86.5385 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCATGGTTGAGGCTCCA |
| Suggested primer 2: | CGCAGCGTTCACGACTTATG |