Insertion junction: CLIP2.000699_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000699
Chromosome:chromosome 9
Location:3173764
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394473 LCI9 Low-CO2-inducible protein; (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCTATACCATCTAAGCCCCTGCTATGTCCGGGACACGAAACCTTCTGC
Internal bar code:TACTTGTTGTTTCTTCGCTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2974
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGATGCCATGAAGAGTTG
Suggested primer 2:GTGTGCGAAGGTTGGTGAAC