Insertion junction: CLIP2.000711_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.000711
Chromosome:chromosome 1
Location:1577727
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g008550 (1 of 2) PTHR24058//PTHR24058:SF23 - DUAL SPECIFICITY PROTEIN KINASE // SERINE/THREONINE KINASE-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTCGAAGCTGGACTGGTCGTACTTGAGCGCCGGCACGCCGGGAGCCAC
Internal bar code:ATAGTACACTATCGCTGGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:105
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCCCACCTCCAGGACTA
Suggested primer 2:CACCAGCCATATCCCCGTAC