| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000741 |
| Chromosome: | chromosome 12 |
| Location: | 1770991 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485850 | CPLD64 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF06916 - Protein of unknown function (DUF1279) (DUF1279) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACAACGCGTAAGAGAGCAGTCTTGTGGTCTTAAGTACAACGTGGTTT |
| Internal bar code: | ATAACCCGGAGACCTACCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1079 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCAGGTTTGCACAGCTG |
| Suggested primer 2: | ACCTGAGGTGTATGTGCAGC |