Insertion junction: CLIP2.000747_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.000747
Chromosome:chromosome 2
Location:6184209
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g120150 RBCS2 (1 of 2) K01602 - ribulose-bisphosphate carboxylase small chain (rbcS); Ribulose-1%252C5-bisphosphate carboxylase/oxygenase small subunit 2, chloroplastic CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCAAATGGAAACGGCGACGCAGGGTTAGATGCTGCTTGAGACAGCGAC
Internal bar code:CTCCTCGCCCCCTACGGCGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:141
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGTGACTATCGTGCGCG
Suggested primer 2:TTGCTTTTCCGTGCTGAAGC