| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000760 |
| Chromosome: | chromosome 8 |
| Location: | 2596937 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373100 | CYP97C3,CYP25 | Cytochrome P450, CYP97 superfamily; (1 of 1) 1.14.99.45 - Carotene epsilon-monooxygenase / LUT1 | outside_mRNA |
| Cre08.g373150 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCTGAGATGCATGCGTACAGCAATTGTGCGTGACGCAATTGAGCTGG |
| Internal bar code: | ACAATCAAGTTCGTGTTTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1909 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACGAGTACGACCATCGC |
| Suggested primer 2: | CTCATATCCTCGCCTGCAGG |