| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.000761 |
| Chromosome: | chromosome 17 |
| Location: | 4061560 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728700 | (1 of 1) K11228 - mitogen-activated protein kinase kinase kinase (STE11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTTCGTCACCGCGCAGCGCTCTAACTCCGGCGGTGGCGGCGGCGGC |
| Internal bar code: | TTATAGTTATCAAAATCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1365 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGTGGCAGTGGTTACGC |
| Suggested primer 2: | GACTCCCCCTTGTAACCGTG |