| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.000769 |
| Chromosome: | chromosome 7 |
| Location: | 3336034 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334900 | (1 of 3) IPR004089 - Methyl-accepting chemotaxis protein (MCP) signalling domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGGCACTACCAGGTCGTTGTAGAGCTTGGGCCCCAGCACGAAGAACA |
| Internal bar code: | TTAGGCGGCATACTACTTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1062 |
| LEAP-Seq percent confirming: | 35.7143 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGAGGTGAAGGCTAGG |
| Suggested primer 2: | TGTTTGGAACACCTGCCTGT |