| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000773 |
| Chromosome: | chromosome 17 |
| Location: | 6734014 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746247 | (1 of 1) K11430 - histone-lysine N-methyltransferase EZH2 (EZH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGACACGGCCGCCAAACCTCTTGATGCCAGTGCGAGACGCCCCGCCCG |
| Internal bar code: | TATTACGCGTTGCGCCAGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAACCCCTGATGTTCGTC |
| Suggested primer 2: | GTATCACTCCCCTCCCTCCA |