| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000786 |
| Chromosome: | chromosome 12 |
| Location: | 7060358 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560250 | (1 of 5) PF04884 - Vitamin B6 photo-protection and homoeostasis (DUF647) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTCGCGTTAGCGCCCAAGCTCGCCACACGTCAATCATGCTCGTTAGAG |
| Internal bar code: | GAGTACGAGTTGAGTGGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1097 |
| LEAP-Seq percent confirming: | 61.2903 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGGTCTTGGATCCTTGG |
| Suggested primer 2: | CAAACTGCGAGTGTTGTGGG |