Insertion junction: CLIP2.000803_5

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.000803
Chromosome:chromosome 13
Location:3850339
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCTGTCCACTGCCTGCCCTCCACACACAGGTTCCGCGCCGTGTTTGGA
Internal bar code:ATGCTGTTCGAAGTGGGAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1409
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAGGACGTAGGTCACCT
Suggested primer 2:CTACATGCAAACGAGCGCTC