Insertion junction: CLIP2.000807_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000807
Chromosome:chromosome 16
Location:638454
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g691800 FLVB1,FLVB (1 of 2) 1.5.3.22 - Coenzyme F420H(2) oxidase; Thylakoid flavodiiron protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCTGACGTGGCGCCTGTTGACCACTCGAGTCCGCGACCCTCCCCACG
Internal bar code:AACATATGCAGGGCTTAAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3851
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGCACCTGTAACTAGCC
Suggested primer 2:TACTCCCTGTGCTACTGCCT