| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000808 |
| Chromosome: | chromosome 17 |
| Location: | 4063082 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728700 | (1 of 1) K11228 - mitogen-activated protein kinase kinase kinase (STE11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACACGATGCAGCTGTTGCTGCTTGAGGTGAGGGGCGGGTGGGGGCGG |
| Internal bar code: | AGTTCAGTCACTCGGTCATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2177 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACCTGCCCAATCATCAG |
| Suggested primer 2: | GCGTAACCACTGCCACTACT |