Insertion junction: CLIP2.000815_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000815
Chromosome:chromosome 13
Location:3851419
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTGTGGCTGGATCCAAACACGGTCATGTTGAAGCGCACGCCGCTGTCC
Internal bar code:GGTATAGAGGAGTGGTCAAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4225
LEAP-Seq percent confirming:87.8378
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGACCTACGTCCTCTCCT
Suggested primer 2:CAGGCTCAGAGCTAACCACC