| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000818 |
| Chromosome: | chromosome 2 |
| Location: | 6183023 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g120150 | RBCS2 | (1 of 2) K01602 - ribulose-bisphosphate carboxylase small chain (rbcS); Ribulose-1%252C5-bisphosphate carboxylase/oxygenase small subunit 2, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCGCTTCAAATACGCCCAGCCCGCCCATGGAGAAAGAGGCCAAAATC |
| Internal bar code: | ATCGCTCCCCTAGTGACTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5261 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 128 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCAGCACGGAAAAGCAA |
| Suggested primer 2: | TCATTGACGAGTGTTGCGGA |