Insertion junction: CLIP2.000827_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000827
Chromosome:chromosome 9
Location:5423538
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g408464 (1 of 1) PF10637 - Oxoglutarate and iron-dependent oxygenase degradation C-term (Ofd1_CTDD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCTCCTTGGCGAATCATGATTGTAACCCCACCTCCCAGCACACTCGCC
Internal bar code:TTCATAACTTCTCGTGGGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:159
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATGACCAAACCCCAACC
Suggested primer 2:CACCTACTCGACCACACACC