Insertion junction: CLIP2.000827_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000827
Chromosome:chromosome 9
Location:5423538
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g408464 (1 of 1) PF10637 - Oxoglutarate and iron-dependent oxygenase degradation C-term (Ofd1_CTDD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGACGGTAGGCAAACCCGGGGGAACAGCAATCATTACCGACGACGTCA
Internal bar code:GAGGACGAGTCATGTGTTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:891
LEAP-Seq percent confirming:34.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTACTCGACCACACACC
Suggested primer 2:GCAATGACCAAACCCCAACC