Insertion junction: CLIP2.000861_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.000861
Chromosome:chromosome 17
Location:5222544
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g737200 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCGAGGTCGGCAACGTCGCAGCACCACAGTGCCACGCACCATCCCACG
Internal bar code:CCAGTCAGTATATGAGCTTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:803
LEAP-Seq percent confirming:10.8108
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTTGGACGTGGACATAA
Suggested primer 2:CAAGTTCTTCGACGACCCCA