| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000866 |
| Chromosome: | chromosome 16 |
| Location: | 2432123 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659950 | PRPS5 | Chloroplast Ribosomal Protein S5; (1 of 2) K02988 - small subunit ribosomal protein S5 (RP-S5, MRPS5, rpsE) | 3'UTR |
| Cre16.g660000 | CMT1A,CPLD63 | (1 of 2) PTHR12608:SF7 - GDT1-LIKE PROTEIN 2, CHLOROPLASTIC; Mn transport into chloroplast | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACAGCGCTGGTCAGGCATGGCTAGCTGCCAGGCAGATACAGCCCGGT |
| Internal bar code: | GTTACACAGATGCGGAGTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1633 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTATCGTGCGCATGTGCA |
| Suggested primer 2: | TTGACGGAATGCGTTGGGTA |