| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000869 |
| Chromosome: | chromosome 8 |
| Location: | 4347614 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384750 | AMYA3,AMA3 | Alpha-amylase 3; (1 of 3) K01176 - alpha-amylase (E3.2.1.1, amyA, malS) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTGAGAACCACGACACGGGCAGCACGCAGCAGCACTGGCCCTTCCCCT |
| Internal bar code: | AAATGGAGATAGTCCTCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2175 |
| LEAP-Seq percent confirming: | 79.0698 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCCACTCACTTCTCTG |
| Suggested primer 2: | ATGCACAAACAACCCATGCC |