| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000872 |
| Chromosome: | chromosome 6 |
| Location: | 6781829 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296000 | PSK1 | (1 of 1) 2.7.1.164 - O-phosphoseryl-tRNA(Sec) kinase / PSTK; Seryl-tRNA(Sec) kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGATGCCGGGCTGTGAGGTGAGCCCGCCGGAAGGGTGTGTGGGGGGG |
| Internal bar code: | GCGAAGTGAGGTGCTATGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3120 |
| LEAP-Seq percent confirming: | 83.908 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTCCGACGCGAACAAGC |
| Suggested primer 2: | TGCACCACTGAAGAGCTCTG |