| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.000880 |
| Chromosome: | chromosome 10 |
| Location: | 4693872 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGTGTTTGGCGGCTGGGTCCTCGGATGGCCGCGCTGCTGTGTACCT |
| Internal bar code: | CGGAAATTAAACTAGGTGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCAACGTCGGACTCTTT |
| Suggested primer 2: | CCCCTCCCTTCATCCCCTAA |