| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.000909 |
| Chromosome: | chromosome 8 |
| Location: | 4344293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384750 | AMYA3,AMA3 | Alpha-amylase 3; (1 of 3) K01176 - alpha-amylase (E3.2.1.1, amyA, malS) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCACACTTGACCTCTAGGTTCGGCCGCGGCCGGGGCTGCCAAGCCTT |
| Internal bar code: | ACGTGTCCTTTGTTCTTTATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCATCGCGACTCTCTGAC |
| Suggested primer 2: | AGACGTGTGTGTATGTGCGT |